Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells

Figure 4 Impact of suberoylanilide hydroxamic acid treatment on the levels of histone H4 lysine 16 acetylation in the promoter region of family with sequence similarity 134 member B in Huh7 and MHCC97L cells. A-C: Huh7 and MHCC97L cells were exposed to 0, 1, 3, 6, and 12 μmol/L suberoylanilide hydroxamic acid for 48 h. Relative protein levels of histone H3 lysine 27 acetylation (H3K27ac), total H4ac, H4K5ac, H4K12ac, and H4K16ac were normalized to total histone H4. Representative western blot images from three independent replicate assays are exhibited; D: Huh7 cells were exposed to 0 μmol/L or 6 μmol/L suberoylanilide hydroxamic acid for 48 h. Quantification of the acetylated histone H4K16-related promoter region of family with sequence similarity 134 member B in Huh7 cells was measured using chromatin immunoprecipitation assay and quantitative polymerase chain reaction. Data are exhibited as mean ± standard deviation. ^aP < 0.05 vs 0 μmol/L group (n = 3). FAM134B: Family with sequence similarity 134 member B; ChIP: Chromatin immunoprecipitation.