Suberoylanilide hydroxamic acid upregulates reticulophagy receptor expression and promotes cell death in hepatocellular carcinoma cells

Figure 2 Impact of suberoylanilide hydroxamic acid treatment on the migration and invasion of Huh7 and MHCC97L cells. A and B: During experimental verification, the mobile ability of hepatocellular carcinoma cells could not be observed in the 6 μmol/L and 12 μmol/L suberoylanilide hydroxamic acid (SAHA) groups and even the cell growth was directly inhibited. Therefore, we selected 3 μmol/L SAHA to treat Huh7 and MHCC97L cells in a transwell assay. Both cell lines were vehicle-treated with 0.1% dimethyl sulfoxide or exposed to 3 μmol/L SAHA for 48 h. The migration and invasion behavior of cells was evaluated by calculating the cells stained using crystal violet. Representative pictures of invaded hepatocellular carcinoma cells from three independent replicate assays are exhibited; C and D: Statistical graphs; E: In the cell scratch experiment, the space in the middle was artificially divided into regions to observe the motility of Huh7 and MHCC97L cells upon treatment with various doses of SAHA (0, 1, 3, 6, and 12 μmol/L) for 48 h. All cells were observed under an inverted microscope with a 40-fold objective lens. Data are exhibited as mean ± standard deviation. ^aP < 0.05 vs control group (n = 3).