Angiotensin-converting enzyme 2 improves liver fibrosis in mice by regulating autophagy of hepatic stellate cells

Figure 6 Effect of rAAV-Angiotensin-converting enzyme 2 treatment on the expression of adenosine monophosphate activates protein kinases signaling pathway proteins and autophagy-related proteins in mouse liver tissues. A: The expression levels of p-AMPK, AMPK and p-mTOR were detected by western blotting; B: The expression levels of autophagy markers Beclin-1, LC3I and LC3II were detected by western blotting; C-F: The relative protein levels in each group were analyzed using ImageJ. ^aP < 0.01 vs Control; ^bP < 0.05 vs CCl₄; ^cP < 0.05 vs rAAV-ACE2 + CCl₄; ^dP < 0.05 vs Control; ^eP < 0.01 vs CCl₄; ^fP < 0.001 vs Control; ^gP < 0.001 vs CCl_4. Rapa: Rapamycin; p-mTOR: p-mammalian target of rapamycin; AMPK: Adenosine monophosphate activates protein kinases; ACE2: Angiotensin-converting enzyme 2.