Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I

Figure 2 Differentiation of dominant hepatitis B virus rtL269 genotype variants using an endpoint genotyping method (LC96 software). L plus I genotype mixtures were prepared with known amounts of the genotypes in various ratios. I-dominant mixtures were positioned closer to axis Hex with higher Hex-fluorescence values, whereas L-dominant ones were located closer to axis FAM with higher FAM-fluorescence values. NTC: Nontemplate control.