Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I

doi:10.3748/wjg.v29.i11.1721

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 21, 2023; 29(11): 1721-1734
Published online Mar 21, 2023. doi: 10.3748/wjg.v29.i11.1721

Table 4 Samples which cannot be identified by locked nucleic acid real-time polymerase chain reaction assay

No.	Patients	Direct sequencing (AAACTCAARCAATGT)	Type	LNA-RT-PCR
1	SNU3 30 HCC	AAAATCAAGCACTGT	I	Not detected
2	SNU3 70 HCC	AAAATTAAGCAATGT	I	Not detected
3	SNU3 82 CH	AAAATCAAACTATGT	I	Not detected
4	SNU3 123 HCC	AAACTTAAGCAATGT	L	Not detected
5	SNU3 31 CH	AAAATCCAGCAATGT	I	Not detected
6	SNU3 355 LC	AAAATTAAGCAATG	I	Not detected
7	SNU3 388 LC	AAACTTAAGCAATGT	L	Not detected

Bases in bold indicate the different ones from the target probe sequence. LNA-RT-PCR: Locked nucleic acid real-time polymerase chain reaction; HCC: Hepatocellular carcinoma; CH: Chronic hepatitis; LC: Liver cirrhosis.

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Citation: Kim K, Choi YM, Kim DH, Jang J, Choe WH, Kim BJ. Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I. World J Gastroenterol 2023; 29(11): 1721-1734
URL: https://www.wjgnet.com/1007-9327/full/v29/i11/1721.htm
DOI: https://dx.doi.org/10.3748/wjg.v29.i11.1721

Kim K, Choi YM, Kim DH, Jang J, Choe WH, Kim BJ. Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269I. World J Gastroenterol 2023; 29(11): 1721-1734 [PMID: 37077521 DOI: 10.3748/wjg.v29.i11.1721]

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