Cystic fibrosis transmembrane conductance regulator prevents ischemia/reperfusion induced intestinal apoptosis via inhibiting PI3K/AKT/NF-κB pathway

Figure 4 Cystic fibrosis transmembrane conductance regulator protected the Caco2 cells from hypoxia/reoxygenation-induced apoptosis by PI3K/AKT/NF-κB pathway. A: Representative images of immunofluorescence staining for NF-κB P65 in Caco2 cells after the LV-cystic fibrosis transmembrane conductance regulator (CFTR) transfection (n = 3); B: Representative western blotting and quantification data for PI3K, p-AKT^ser473, p-AKT^Thr308, and NF-κB P65 after LV-CFTR transfection, ^aP < 0.01 and ^bP < 0.001 vs control (n = 3); C: Cell viability was tested using the CCK8 kit after LV-CFTR transfection and treatment with NF-κB activator Betulinic acid (BA), AKT inhibitor GSK690693 and PI3K inhibitor LY294002, ^bP < 0.001 vs control (n = 3); D: Representative Western blotting and quantification data for cell apoptotic proteins Bcl-2 and Bax after LV-CFTR transfection and treatment with NF-κB activator BA, AKT inhibitor GSK690693 and PI3K inhibitor LY294002. Data are presented as mean ± SD. ^bP < 0.001 vs control (n = 3).