Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1

Figure 8 Alcohol consumption influenced the protein and mRNA levels of epithelial mesenchymal transformation -associated markers in colorectal cancer patients. The bars represent the mean ± SD (n = 8). A: Representative immunohistochemical images of phosphorylate-focal adhesion kinase (p-FAK), FAK, snail, fibronectin, E-cadherin, N-cadherin and special AT-rich sequence binding protein 1 (SATB1) in different groups in clinical samples (× 400); B: Quantification of the protein levels of FAK, p-FAK/FAK, snail, fibronectin, E-cadherin, N-cadherin and SATB1 by counting the average optical density in clinical samples; C: Quantification of the mRNA levels of FAK, snail, fibronectin, E-cadherin, N-cadherin and SATB1 by quantitative real-time polymerase chain reaction in clinical samples. Compared with the non-alcohol group: ^bP < 0.01, ^aP < 0.05. SATB1: Special AT-rich sequence binding protein 1; FAK: Focal adhesion kinase; p-FAK: Phosphorylate focal adhesion kinase; AOD: Average optical density. NP: No statistical difference.