Alcohol promotes epithelial mesenchymal transformation-mediated premetastatic niche formation of colorectal cancer by activating interaction between laminin-γ2 and integrin-β1

Table 1 Primer sequences for quantitative real-time polymerase chain reaction

Gene name	Forward (5’>3’)	Reverse (5’>3’)
For human
LAMC2	TTCTACAACGATCCGCACGAC	GAGTTAAAAGCAGCCCTGGT
ITGB1	CCTACTTCTGCACGATGTGATG	CCTTTGCTACGGTTGGTTACATT
FAK	GTCTGCCTTCGCTTCACG	GAATTTGTAACTGGAAGATGCAAG
Snail	CTAGCGAGTGGTTCTTCTGC	GTAGTTAGGCTTCCGATTGGG
E-cadherin	GACCGGTGCAATCTTCAAA	TTGACGCCGAGAGCTACAC
N-cadherin	AGCTTCTCACGGCATACACC	GTGCATGAAGGACAGCCTCT
Fibronectin	GCCTGGTACAGAATA TGTAGTG	ATCCCAGCTGATC AGTAGGCTGGTG
SATB1	GTGGAAGCCTTGGGAATCC	CTGACAGCTCTTCTTCTAGTT
GAPDH	GGAGCGAGATCCCTCCAAAAT	GGCTGTTGTCATACTTCTCATGG
For rats
LAMC2	GTGAAGCTTCCCTGCAAAAC	CATTTGGCCCCACGTAGT
ITGB1	TCGAAGGACCACTAGACCTGA	TTCCATGGGAACAAAAGGTAA
FAK	TTGGGTTGCAAACTATCTCTAGAAC	TGGTACAAAACTGGCACAGAA
Snail	TTCACATCCGAGTGGGTCTG	ACCCACACTGGTGAGAAGCC
E-cadherin	AAAGCAGGAAGAAAACACCACTC	AAAGGGCACGCTATCAACATTAG
N-cadherin	TCAGTGGCGGAGATCCTAC	GTGCTGAATTCCCTTGGCTA
Fibronectin	TGTCACCCACCACCTTGA	CTGATTGTTCTTCAGTGCGA
SATB1	AGATGCAGGGAGTGCCTTTA	TGCTCCTCCTTGCAATCATA
GAPDH	TGCCCTCATGTTCCTGATAAAT	CATTACATCACAGCTTTCCAGG

ITGB1: Integrin-β1; LAMC2: Laminin-γ2; SATB1: Special AT-rich sequence binding protein 1; FAK: Focal adhesion kinase; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.