Immunological mechanisms of fecal microbiota transplantation in recurrent Clostridioides difficile infection

Figure 1 Advantages and disadvantages of non-classical preparations methods of donor’s fecal material prior to fecal microbiota transplantation. ¹Classical preparation consist in dissolve donor’s fecal material by blending with saline water and filter out residual solid feces through gauze or fabric. ²Isolation of different bacteria strains directly from donor’s fecal material. ³Basically, this method consists in consecutively centrifugation of microbiota from donors to remove the supernadants. ⁴Uses filtration systens to retain debris and bacterial load from the donor’s fecal material. SCFAs: Short-chain fatty acids; AMPs: Antimicrobial peptides; BAs: Bile acids.