Involvement of toll-like receptor 5 in mouse model of colonic hypersensitivity induced by neonatal maternal separation

Figure 3 Neonatal maternal separation paradigm induces alterations of core fecal microbiota related to colonic hypersensitivity. A: Alpha-diversity analysis of the core microbiota. Number of observed operational taxonomic units according to the number of sequences per samples of fecal samples from non-handled (NH), neonatal maternal separated non-sensitized (NMS NS) and neonatal maternal separated sensitized (NMS S) at week 3 (W3), week 4 (W4) and week 12 (W12); B: Beta-diversity analysis of the core microbiota. Principal coordinates analysis (PCoA) of unweighted UniFrac distances of NH, NMS NS and NMS S mice at W3, W4 and W12; C and D: Mean relative abundances of bacterial phyla (C) and genera (D) significantly altered by the NMS paradigm between NH, NMS NS and NMS S mice at W12. NH: n = 6; NMS NS: n = 6; NMS S: n = 8. ^aP < 0.05, ^bP < 0.01 and ^cP < 0.001 vs NH or ^dP < 0.05, ^eP < 0.01 and ^fP < 0.001 vs NMS NS groups respectively. For alpha-diversity analysis, dots represent means and error bars represent SEM. For PCoA analysis each dot represents one mouse.