Comprehensive evaluation of microRNA as a biomarker for the diagnosis of hepatocellular carcinoma

Table 2 Individual standard deviation and accumulated standard deviation for consecutive microRNA reference genes

miRNA	SD	SDacc
hsa-let-7i-5p	0.49	0.49
hsa-miR-222-3p	0.57	0.37
hsa-miR-23a-3p	0.63	0.33
hsa-miR-30e-5p	0.65	0.29
hsa-miR-191-5p	0.79	0.28

The lower the standard deviation/accumulated standard deviation, the more applicable microRNA/microRNA combination was as reference gene. These miRNAs were used as endogenous controls for the normalization of real-time quantitative PCR data. SD: Standard deviation; SD_acc: Accumulated standard deviation; miRNA: MicroRNA; miR: MicroRNA.