Intracellular alpha-fetoprotein mitigates hepatocyte apoptosis and necroptosis by inhibiting endoplasmic reticulum stress

doi:10.3748/wjg.v28.i26.3201

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Jul 14, 2022 (publication date) through Apr 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastroenterol. Jul 14, 2022; 28(26): 3201-3217
Published online Jul 14, 2022. doi: 10.3748/wjg.v28.i26.3201

Table 2 The sequences of short hairpin RNAs

Insert content			5'-3'
Mouse	Afp shRNA	Target sequence	GCATCCATTGCAAAGGAATTA
	Afp shRNA	shRNA sequence	GCATCCATTGCAAAGGAATTACGAATAATTCCTTTGCAATGGATGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAACGAATTCTCCGAACGTGTCACGTTT
Human	PERK shRNA	Target sequence	GCACTTTAGATGGGAGAATTG
	PERK shRNA	shRNA sequence	GCACTTTAGATGGGAGAATTGCGAACAATTCTCCCATCTAAAGTGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAACGAATTCTCCGAACGTGTCACGTTT
	ATF6 shRNA	Target sequence	GCAGGTCCTCCTGTTATTAGA
	ATF6 shRNA	shRNA sequence	GCAGGTCCTCCTGTTATTAGACGAATCTAATAACAGGAGGACCTGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAA CGAATTCTCCGAACGTGTCACGTTT
	AFP shRNA	Target sequence	GCTTCCATATTGGATTCTTAC
	AFP shRNA	shRNA sequence	GCTTCCATATTGGATTCTTAC CGAAGTAAGAATCCAATATGGAAGC
	Control shRNA	shRNA sequence	AAACGTGACACGTTCGGAGAA CGAATTCTCCGAACGTGTCACGTTT

AFP: Alpha-fetoprotein; ATF6: Activating transcription factor-6; PERK: Protein kinase R-like ER kinase; shRNA: Short hairpin RNA.

Citation: Chen YF, Liu SY, Cheng QJ, Wang YJ, Chen S, Zhou YY, Liu X, Jiang ZG, Zhong WW, He YH. Intracellular alpha-fetoprotein mitigates hepatocyte apoptosis and necroptosis by inhibiting endoplasmic reticulum stress. World J Gastroenterol 2022; 28(26): 3201-3217
URL: https://www.wjgnet.com/1007-9327/full/v28/i26/3201.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i26.3201