Fecal gene detection based on next generation sequencing for colorectal cancer diagnosis

Table 3 Summary of gene mutations in colorectal cancer patients

Gene	Tumor tissue		Preoperative feces		Postoperative stools
	Total number of mutations	Mutation frequency	Total number of mutations	Mutation frequency	Total number of mutations	Mutation frequency
TP53	40	62.00% (31/50)	27	59.26% (16/27)	7	31.58% (6/19)
APC	15	24.00% (12/50)	3	11.11% (3/27)	0	0
KRAS	30	58.00% (29/50)	11	37.04% (10/27)	0	0
PIK3CA	13	22.00% (11/50)	1	3.70% (1/27)	2	10.53% (2/19)
FBXW7	5	10.00% (5/50)	1	3.70% (1/27)	0	0
GNAS	2	4.00% (2/50)	1	3.70% (1/27)	0	0
PTEN	11	22.00% (11/50)	1	3.70% (1/27)	0	0
ABL1	0	0	1	3.70% (1/27)	0	0
PDGFRA	12	24.00% (12/50)	8	29.63% (8/27)	6	31.58% (6/19)
ATM	1	2.00% (1/50)	0	0	0	0
SMAD4	1	2.00% (1/50)	0	0	0	0
BRAF	2	4.00% (2/50)	0	0	2	10.53% (2/19)
PTPN11	1	2.00% (1/50)	0	0	0	0
NRAS	1	2.00% (1/50)	0	0	0	0
CTNNB1	3	6.00% (3/50)	1	3.70% (1/27)	0	0
STK11	2	4.00% (2/50)	1	3.70% (1/27)	0	0
AKT1	1	2.00% (1/50)	1	3.70% (1/27)	0	0
CDKN2A	3	6.00% (3/50)	4	7.41% (2/27)	3	5.26% (1/19)
HRAS	21	42.00% (21/50)	12	40.74% (11/27)	8	42.11% (8/19)
EGFR	2	4.00% (2/50)	2	7.41% (2/27)	1	5.26% (1/19)
IDH1	2	4.00% (2/50)	2	7.41% (2/27)	1	5.26% (1/19)
NOTCH1	2	4.00% (2/50)	0	0	2	5.26% (1/19)
VHL	0	0	1	3.70% (1/27)	0	0
KIT	7	14.00% (7/50)	2	7.41% (2/27)	4	21.05% (4/19)
MET	9	18.00% (9/50)	5	18.52% (5/27)	4	21.05% (4/19)
MLH1	3	6.00% (3/50)	2	7.41% (2/27)	2	10.53% (2/19)
MPL	0	0	0	0	1	5.26% (1/19)

Total number of mutations: The overall number of mutations of any gene in various samples. Mutation frequency: The number of people with mutations in various samples/total number of people.