Loss of LAT1 sex-dependently delays recovery after caerulein-induced acute pancreatitis

Figure 3 The trypsin activation observed in LAT1-ko mice had higher variability, and the expression of trypsinogens and cathepsins differed from LAT1-wt mice. A: Trypsin activation was observed in LAT1-wt and LAT1-ko mice 24 h after acute pancreatitis (AP) induction. The activity was measured using Boc-Q-A-R-MCA and expressed as nmol/μg DNA. Mean ± SEM, n = 3-7; B-C: The mRNA expression of trypsinogens, trypsin inhibitor, and cathepsins. The mRNA expressions of serine protease 1+3 (prss1+3) and serine protease 2 (prss2) along with serine peptidase inhibitor kazal type 1 (spink1) (B) and cathepsin B, D, and L (ctsb, ctsd, and ctsl) (C) were analyzed via qPCR and expressed relative to a housekeeping gene (18S × 10⁶) in LAT1-wt and LAT1-ko mice. The upper panel depicts the mice that were injected with saline, and the lower panel shows the mice 24 h after AP induction. Mean ± SEM, n = 7-14. Statistical analysis was conducted with ANOVA and Bonferroni and compared, as indicated by the capped line. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.