Loss of LAT1 sex-dependently delays recovery after caerulein-induced acute pancreatitis

doi:10.3748/wjg.v28.i10.1024

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World Journal of Gastroenterology

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1007-9327

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World J Gastroenterol. Mar 14, 2022; 28(10): 1024-1054
Published online Mar 14, 2022. doi: 10.3748/wjg.v28.i10.1024

Table 2 Antibodies and conditions applied in Western blotting experiments

Antigen	Total protein loaded (µg)	Primary antibody	Antibody dilution
Amylase	2.5	LifeSpan BioSciences, Inc. (LS-B8239)	1:10000
β-tubulin	Varying	Sigma (T8328)	1:5000
LAT1	20	Cosmobio (KE026)	1:500
LC3	100	MLB (PM036)	1:250
NA/K-ATPase	Varying	Santa Cruz (sc-48345)	1:10000
p62	50	MBL (PM045)	1:1000
peIF2α	25	Cell Signaling (3398)	1:1000
pS6K	50	Cell Signaling (9205S)	1:1000
SMA	25	Sigma (C6198)	1:1000
teIF2α	25	Cell Signaling (9722)	1:1000
tS6K	50	Cell Signaling (2708P)	1:1000
Vimentin	30	Invitrogen (MA5-11883)	1:1000

LC3: Microtubule-associated proteins 1A/1B light chain 3B; peIF2: Phosphorylated eukaryotic translation initiation factor 2; pS6K: Phosphorylated S6 kinase; SMA: Smooth muscle actin; teIF2α: Total eukaryotic translation initiation factor 2α; tS6K: Total S6 kinase.

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Citation: Hagen CM, Roth E, Graf TR, Verrey F, Graf R, Gupta A, Pellegrini G, Poncet N, Camargo SMR. Loss of LAT1 sex-dependently delays recovery after caerulein-induced acute pancreatitis. World J Gastroenterol 2022; 28(10): 1024-1054
URL: https://www.wjgnet.com/1007-9327/full/v28/i10/1024.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i10.1024

Hagen CM, Roth E, Graf TR, Verrey F, Graf R, Gupta A, Pellegrini G, Poncet N, Camargo SMR. Loss of LAT1 sex-dependently delays recovery after caerulein-induced acute pancreatitis. World J Gastroenterol 2022; 28(10): 1024-1054 [PMID: 35431492 DOI: 10.3748/wjg.v28.i10.1024]

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