Focal adhesion kinase-related non-kinase ameliorates liver fibrosis by inhibiting aerobic glycolysis via the FAK/Ras/c-myc/ENO1 pathway

Figure 5 FRNK does not directly target ENO1. A and B: Proteins were extracted from LX-2 cells, and relative levels of the pY397-FAK, K-Ras, c-myc and ENO1 proteins were determined; C and D: After transfection of LX-2 cells with pcDNA-c-myc or pcDNA-vector (NC), adenoviral vectors containing the FRNK gene (Ad-FRNK) or the negative control (Ad-GFP) were transfected, and the extracted protein was used to evaluate ENO1 expression through Western blotting; E: Schematic representation of the structure of the putative c-myc binding site in the human ENO1 promoter and chromatin immunoprecipitation (ChIP) assays with anti-c-myc or IgG; F: A dual-luciferase reporter assay showed the luciferase activity of WT, mutation (MT)1 and MT2 ENO1 promoters in LX-2 cells transfected with the c-myc or NC plasmid. Representative results from three independent replicate assays are shown. ^aP < 0.05 and ^bP < 0.01. Results are presented as the mean ± SD.