Abdominal paracentesis drainage attenuates intestinal inflammation in rats with severe acute pancreatitis by inhibiting the HMGB1-mediated TLR4 signaling pathway

Figure 4 Effects of abdominal paracentesis drainage on intestinal toll-like receptor 4 signaling pathway in rats with severe acute pancreatitis. A: Representative immunohistochemistry images of toll-like receptor 4 (TLR4) in intestinal tissue (bar = 100 μm); B: TLR4 mRNA measurement by real-time polymerase chain reaction (PCR); C: Immunoblotting of TLR4, myeloid differentiation factor 88 (MyD88) and p65 protein expression and p65 phosphorylation level from intestinal samples; D-F: Densitometry analysis of TLR4, MyD88 and ratio of p65 to phosphorylated p65. Data are expressed as means ± SD, n = 6 real-time PCR results per group; means ± SD, n = 3 immunoblotting results per group. ^aP < 0.05 vs sham group; ^bP < 0.05 vs severe acute pancreatitis group. SAP: Severe acute pancreatitis; APD: Abdominal paracentesis drainage; MyD88: Myeloid differentiation factor 88; TLR4: Toll-like receptor 4; p-p65: Phosphorylated p65; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.