Abdominal paracentesis drainage attenuates intestinal inflammation in rats with severe acute pancreatitis by inhibiting the HMGB1-mediated TLR4 signaling pathway

Figure 3 Effects of abdominal paracentesis drainage on intestinal inflammation and mucosal cell apoptosis induced by severe acute pancreatitis. A: Intestinal tumor necrosis factor-α; B: Intestinal interleukin-6; C: Intestinal myeloperoxidase activity; D: Representative images (400 × magnification) of terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL) assay; E: Statistical results of the number of apoptotic intestinal mucosal cells in each group; F: Immunoblot of Bax, Bcl-2, pro-caspase-3 and cleaved-caspase-3 protein expression from intestinal samples; G-I: Densitometry analysis of the immunoblot data of apoptosis-related proteins in intestinal tissue. Data are expressed as means ± SD, n = 6 enzyme-linked immunosorbent assay and TUNEL assay results per group; means ± SD, n = 3 immunoblot results per group. ^aP < 0.05 vs sham group; ^bP < 0.05 vs severe acute pancreatitis group. TNF-α: Tumor necrosis factor-α; IL-6: Interleukin-6; MPO: Myeloperoxidase; SAP: Severe acute pancreatitis; APD: Abdominal paracentesis drainage; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.