Demethylation of miR-34a upregulates expression of membrane palmitoylated proteins and promotes the apoptosis of liver cancer cells

Figure 3 Upregulation of microRNA 34a promotes cell apoptosis and membrane palmitoylated protein expression, and inhibits cell proliferation, invasion, and migration. A: Image of microRNA 34a (miR-34a) demethylation results; B: Upregulation of miR-34a inhibits cell migration; a indicates that compared with negative control (NC) demethylation group, ^aP < 0.05; ^bP < 0.01, and d indicates compared with the NC pre group, ^dP < 0.05; C: miR-34a upregulated the number of cell invasions; a indicates compared with the NC demethylation group, ^aP < 0.05; d indicates compared with the NC pre group, ^dP < 0.05; D: miR-34a upregulated and inhibited cell activity; a indicates ^aP < 0.05, ^bP < 0.01; E: Upregulation of miR-34a inhibited the downregulation of B-cell lymphoma 2 (Bcl-2), N- cadherin and β-catenin expression, while promoting caspase 9, caspase 3, E-cadherin and Bcl-2-associated X protein; a indicates compared with the NC demethylation group, ^aP < 0.05; and d indicates compared with the NC pre group, ^dP < 0.05; and F: Upregulation of miR-34a promoted the expression of membrane palmitoylated proteins, c indicates ^cP < 0.001.