MTNR1B polymorphisms with CDKN2A and MGMT methylation status are associated with poor prognosis of colorectal cancer in Taiwan

Table 5 Stratified effect between gene promoter region methylation and MTNR1B genotypes for 5-year overall survival of colorectal cancer patients

		No. of subjects	No. of cases (%)	Crude		Adjusteda
				HR	95%CI	HR	95%CI
rs1387153 (C>T)
TT vs CC + CT	CDKN2A
	U	10	4 (40.0)	5.01	(1.33 to 18.9)	10.4	(1.17 to 92.4)
	M	11	4 (36.4)	3.83	(0.96 to 15.3)	8.86	(1.08 to 72.8)
	MGMT
	U	12	5 (41.7)	3.63	(1.05 to 12.6)	8.57	(1.67 to 44.1)
	M	9	3 (33.3)	4.93	(1.10 to 22.1)	3.05	(0.33 to 28.0)
rs2166706 (T>C)
CC vs TT + TC	CDKN2A
	U	10	4 (40.0)	5.02	(1.33 to 19.0)	10.4	(1.17 to 92.4)
	M	13	5 (38.5)	3.96	(1.06 to 14.7)	10.1	(1.49 to 68.0)
	MGMT
	U	13	5 (38.5)	3.07	(0.89 to 10.7)	6.28	(1.54 to 25.7)
	M	23	9 (39.1)	6.25	(1.56 to 25.1)	8.28	(0.95 to 72.3)
rs10830963 (C>G)
GG vs CC + CG	CDKN2A
	U	13	5 (38.5)	4.63	(1.24 to 17.3)	8.47	(1.57 to 45.6)
	M	10	5 (50.0)	5.61	(1.51 to 20.9)	27.2	(3.12 to 233)
	MGMT
	U	12	5 (41.7)	3.47	(1.00 to 12.0)	8.50	(1.98 to 36.5)
	M	11	5 (45.5)	7.91	(1.89 to 33.2)	9.80	(1.42 to 67.5)
rs1447352 (A>G)
AA vs GG + GA	CDKN2A
	U	22	6 (27.3)	2.05	(0.51 to 8.22)	2.28	(0.51 to 10.2)
	M	28	8 (28.6)	7.34	(0.92 to 58.7)	9.40	(1.02 to 86.8)
	MGMT
	U	24	8 (33.3)	3.94	(0.84 to 18.6)	19.4	(2.94 to 128)
	M	26	6 (23.1)	2.77	(0.56 to 13.7)	2.04	(0.35 to 12.0)

^aAdjusted for age, sex, stage, adjuvant chemotherapy, tumor location and the methylation status of CDKN2A and MLH1 or MGMT and MLH1 gene. U: Unmethylation; M: Methylation; HR: Hazard ratio.