Ubiquitin-specific protease 15 contributes to gastric cancer progression by regulating the Wnt/β-catenin signaling pathway

Figure 4 Ubiquitin-specific protease 15 regulates the Wnt/β-catenin pathway in gastric cancer. A: Western blotting confirmed the transfection efficiency of ubiquitin-specific protease 15 (USP15) knockdown in BGC-823 cell lines before RNA sequencing analysis; B: Heat map and hierarchical clustering based on the most differentially expressed genes (5739); C: Volcano plot illustrated differentially regulated gene expression. 1134 upregulated genes (red) and 1209 downregulated genes (green); D: Gene Ontology enrichment analyses of targets associated with USP15 for biological process, cellular component, and molecular function (top 10 most significantly affected categories are shown); E: Expression levels of USP15, β-catenin, c-Myc, and cyclin D1 in BGC-823 and MKN-45 cell lines transfected with USP15 knockdown or SGC-7901 cell lines transfected with USP15 overexpression; F: Immunofluorescence staining of β-catenin in BGC-823 and MKN-45 cell lines transfected with USP15 knockdown. Blue: DAPI; Red: β-catenin. Bar = 50 μm.