Reactive oxygen species-induced activation of Yes-associated protein-1 through the c-Myc pathway is a therapeutic target in hepatocellular carcinoma

Figure 6 The reactive oxygen species-induced oncogenic action of yes-associated protein-1 in hepatocellular carcinoma cells led to an enhanced unfolded protein response. A: Yes-associated protein-1 (YAP-1) small interfering RNA (siRNA) significantly decreased the mRNA expression of unfolded protein response markers, including 78-kDa (glucose-regulated protein 78/BiP), activating transcription factor-6, and XBP1, in reactive oxygen species (ROS)-exposed hepatocellular carcinoma (HCC) cells (P < 0.05). The experiment was repeated three times. The data are expressed as the mean ± SD; B: Immunoblot analyses of YAP-1 and phosphorylated-eIF-2α were performed in ROS-exposed HCC cells transfected with YAP-1 siRNA or control siRNA. The experiment was repeated three times. GRP78: Glucose-regulated protein 78; ATF-6: Activating transcription factor-6; YAP: Yes-associated protein; siRNA: Small interfering RNA.