Reactive oxygen species-induced activation of Yes-associated protein-1 through the c-Myc pathway is a therapeutic target in hepatocellular carcinoma

Figure 3 Modulation of yes-associated protein-1 in reactive oxygen species-exposed hepatocellular carcinoma cells showed antitumor effects in an in vivo xenograft tumor mouse model. A: H₂O₂ treatment significantly increased the proliferation of MH134 cells based on the MTS assay results (P < 0.05). The data are expressed as the mean ± SD. The experiment was repeated three times; B: Yes-associated protein-1 (YAP-1) small interfering RNA (siRNA) transfection significantly suppressed YAP-1 mRNA expression compared to control siRNA transfection in reactive oxygen species-exposed MH134 cells (P < 0.05). The data are expressed as the mean ± SD. The experiment was repeated three times; C: In the xenograft tumor model, the YAP-1 siRNA group showed significantly suppressed tumor growth compared to the control siRNA group at days 11, 12, and 13 after tumor budding (all ^aP < 0.05). The data are expressed as the mean ± SD. YAP: Yes-associated protein; siRNA: Small interfering RNA.