Antioxidant activity and hepatoprotective effect of 10 medicinal herbs on CCl4-induced liver injury in mice

Table 4 Identification of the main phytochemical components in Sanguisorba officinalis decoction using high-performance liquid chromatography-electrospray ionization source-ion trap tandem mass spectrometry (positive or negative mode)

Peak	RT (min)	λmax (nm)	[M+H]+	[M-H]-	MS/MS fragments	MW	Putative compound	Ref.
1	7.10	270	/	169	125	170	Gallic acid1	[33]
2	10.52	275	/	345; 865	313, 169, 151, 125; 739, 695, 577, 543, 407, 287	346; 866	Galloyl-methylglucoside; Procyanidin C2	[33]
3	16.10	278	291	/	273, 165, 151, 139, 123	290	(+)-Catechin1	[33]
4	20.87	275	/	729	577, 559, 407	730	Procyanidin B3 3-O-gallate	[33]
5	22.35	274	563	/	545, 423, 411, 435, 393, 271	562	Fisetinidol-(4α/β→8)-(+)-catechin	[33]
6	23.65	272	/	1103	1059, 935, 633, 469	1104	Sanguiin H-2	[31]
7	54.30	264/362	/	277	197, 182, 111	278	Methoxygallic acid methyl ester 5-O-sulfate	[32]
8	68.30	243/372	345	/	330, 313	344	3,3’,4’-O-trimethylellagic acid	[33]

¹Identification of phytochemical compounds by high-performance liquid chromatography, mass spectrometry (MS), and MS/MS using commercial standards or based on published values[31-33]. RT: Retention time; λmax: Maximum absorbance wavelength; MS: Mass spectrometry; MW: Molecular weight.