Rno_circ_0005139 regulates apoptosis by targeting Wnt5a in rat anorectal malformations

Figure 2 Knockdown and overexpression of rno_circ_0005139 affected proliferation and apoptosis of intestinal epithelial cells. A: Expression of rno_circ_0005139 was detected after transfection with si-rno_circ_0005139 (si-circ), pLO5-rno_circ_0005139 (over-circ), or a negative control (si-NC/vector) in intestinal epithelial cells (IECs), as determined by quantitative reverse transcription polymerase chain reaction; B: Cell Counting Kit-8 assays were used to measure the viability of IECs after transfection (rno_circ_0005139); C and D: Apoptosis detection using flow cytometry was conducted to measure apoptosis in IECs after transfection (rno_circ_0005139). ^aP < 0.01 vs si-5139-NC, ^bP < 0.05 vs si-5139-NC, ^cP < 0.05 vs over-5139-NC.