Optimal dosing time of Dachengqi decoction for protection of extrapancreatic organs in rats with experimental acute pancreatitis

Table 2 Concentrations of the ten major components of Dachengqi decoction in tissue samples (n = 6)

Parameter	SOG1	MG1	MG2	MG3
Heart
Emodin	3.21 ± 0.13	1.25 ± 0.09ad	2.24 ± 0.14c	2.45 ± 0.21
Aloe-emodin	56.72 ± 5.67	21.96 ±7.21ad	28.45 ± 8.47	31.53 ± 1.34
Rhein	0	0	0	0
Chrysophanol	15.57 ± 4.63	6.43 ± 1.2a	5.65 ± 2.4	7.59 ± 1.98
Rheochrysidin	1.93 ± 0.89	0	0	2.41 ± 3.67
Naringin	40.36 ± 1.38	0.85 ± 0.02ad	9.62 ± 0.37c	10.49 ± 0.21
Naringenin	420.68 ± 67.81	160.09 ± 12.61a	924.79 ± 34.67c	270.25 ± 48.51
Hesperidin	12.22 ± 0.94	5.05 ± 1.2a	10.33 ± 4.3 c	5.24 ± 1.98
Magnolol	0.21 ± 0.04	0.18 ± 0.02	0.02 ± 0.01 c	0.21 ± 0.10
Honokiol	0.59 ± 0.07	0.24 ± 0.04ad	0.9 0 ± 0.07 c	0.73 ± 0.09
Liver
Emodin	14.24 ± 6.21	7.27 ± 2.37a	3.97 ± 1.67 c	8.36 ± 4.23
Aloe-emodin	506.09 ± 32.78	438.4 ± 123.47	122.87 ± 79.23 c	383.40 ± 101.31
Rhein	45.58 ± 63.23	0	0	0
Chrysophanol	132.65 ± 7.43	40.38 ± 20.81ad	17.10 ± 3.24 c	18.47 ± 0.8
Rheochrysidin	20.27 ± 0.98	1.42 ± 0.14ad	3.39 ± 0.29 c	0.18 ± 0.23
Naringin	148.20 ± 8.94	13.19 ± 10.23a	27.07 ± 9.81	23.49 ± 8.67
Naringenin	1707.40 ± 99.46	1459.04 ± 98.67ad	692.88 ± 102.43c	1855.76 ± 79.23
Hesperidin	60.03 ± 18.87	6.53 ± 1.23ad	2.27 ± 0.94c	13.65 ± 0.23
Magnolol	2.88 ± 0.41	3.34 ± 1.64	1.62 ± 0.72 c	2.42 ± 0.23
Honokiol	3.00 ± 0.78	2.02 ± 0.78	1.64 ± 0.24	3.03 ± 0.31
Lung
Emodin	3.87 ± 1.43	2.08 ± 0.98	2.99 ± 0.43	3.1 ± 1.02
Aloe-emodin	66.98 ± 33.62	56.88 ± 20.45d	34.94 ± 13.67 c	88.12 ± 23.21
Rhein	143.74 ± 73.64	164.27 ± 64.81	72.59 ± 42.31 c	171.52 ± 57.84
Chrysophanol	19.05 ± 6.39	13.51 ± 9.43	15.1 ± 3.57	16.42 ± 4.81
Rheochrysidin	1.06 ± 0.17	0.18 ± 0.23a	0.42 ± 0.02c	0.23 ± 0.01
Naringin	12.55 ± 1.67	6.00 ± 3.2ad	8.37 ± 2.37c	24.46 ± 4.17
Naringenin	422.09 ± 203.92	601.12 ± 176.64	567.53 ± 123.67	427.36 ± 204.81
Hesperidin	58.67 ± 15.81	22.74 ± 10.13ad	33.74 ± 10.32c	77.35 ± 34.61
Magnolol	0.34 ± 0.19	0.35 ± 0.23	0.35 ± 0.04	0.46 ± 0.17
Honokiol	1.43 ± 0.19	1.42 ± 0.16a	0.95 ± 0.02 c	0.82 ± 0.04
Kidney
Emodin	21.15 ± 10.28	27.75 ± 14.21	11.98 ± 5.67 c	26.31 ± 13.42
Aloe-emodin	1328.18 ± 159.73	279.04 ± 100.04ad	133.62 ± 92.43c	720.74 ± 143.67
Rhein	154.59 ± 12.34	2.49 ± 0.98ad	37.72 ± 1.34c	101.55 ± 2.78
Chrysophanol	108.89 ± 7.64	101.18 ± 7.84ad	46.84 ± 6.23c	124.76 ± 4.32
Rheochrysidin	16.25 ± 2.79	15.23 ± 3.54	6.13 ± 0.98c	13.57 ± 1.43
Naringin	208.08 ± 8.94	18.44 ± 3.64ad	0.94 ± 0.17c	59.9 ± 7.63
Naringenin	2144.99 ± 147.81	2506.95 ± 100.07ad	2360.21 ± 143.64c	1939.2 ± 127.68
Hesperidin	48.87 ± 4.81	9.13 ± 1.67a	8.67 ± 1.32	9.90 ± 0.89
Magnolol	0.81 ± 0.2	0.52 ± 0.12ad	0.30 ± 0.03 c	1.14 ± 0.14
Honokiol	2.76 ± 0.31	1.56 ± 0.44ad	1.35 ± 0.23c	2.63 ± 0.67
Intestine
Emodin	94.43 ± 37.23	19.73 ± 6.72ad	88.3 ± 24.23	122.66 ± 72.61
Aloe-emodin	4515.56 ± 342.67	1447.33 ± 109.73ad	975.33 ± 152.62c	2202.32 ± 143.76
Rhein	214.49 ± 56.72	830.8 ± 48.63ad	130.67 ± 65.67	209.09 ± 100.3
Chrysophanol	714.88 ± 300.68	290.97 ± 110.37ad	410.67 ± 203.3c	736.46 ± 45.67
Rheochrysidin	161.78 ± 80.93	37.9 ± 2.34ad	27.59 ± 17.61c	191.14 ± 59.67
Naringin	2771.61 ± 67.81	604.19 ± 134.6ad	678.67 ± 57.63c	1442.36 ± 100.75
Naringenin	1804.34 ± 307.16	1365.3 ± 153.62ad	1077.59 ± 174.63 c	3695.62 ± 483.54
Hesperidin	1156.54 ± 154.63	241.58 ± 72.61ad	265.59 ± 100.32 c	581.04 ± 201.63
Magnolol	17.13 ± 7.34	18.78 ± 6.43d	18.15 ± 7.81c	35.5 ± 5.67
Honokiol	18.7 ± 2.71	24.27 ± 2.43ad	17.14 ± 3.1c	45.48 ± 4.58

Rats were randomly divided into SOG₁ and three model groups (MG_1, MG_2, and MG₃), and orally dosed with Dachengqi decoction (DCQD) (10 g/kg). Tissue samples were collected 24 h after a single dose to detect its main components. SOG₁: Sham-operated group with the dosing time at 4 h after operation; MG₁, MG₂, and MG₃: Rats were dosed orally with DCQD at 4 h, 12 h, and 24 h after AP induction, respectively. Data are presented as the mean ± SD (n = 6). MG₁ vs SOG₁.

^aP < 0.05; MG₂ vs MG₁. ^bP < 0.05; MG₃ vs MG₂.

^cP < 0.05; MG₃ vs MG₁.

^dP < 0.05.