Optimal dosing time of Dachengqi decoction for protection of extrapancreatic organs in rats with experimental acute pancreatitis

Table 1 Pharmacokinetic parameters of the eight components of Dachengqi decoction in serum (n = 6)

Parameter	SOG1	MG1	MG2	MG3
Emodin
AUC 0-t	1605.0 ± 1029.5	359.3 ± 187.1a	362.5 ± 67.4	427.5 ± 299.3
T 1/2z	5.2 ± 1.4	18.9 ± 12.5a	9.0 ± 3.2	22.6 ± 9.0
T max	0.6 ± 0.3	3.5 ± 1.6a	3.5 ± 1.6	0.3 ± 0dc
C max	620.733 ± 477.0	34.1 ± 22.5a	29.8 ± 8.8	125.2 ± 90.9dc
Aloe-emodin
AUC 0-t	19206.1 ± 7275.8	11860.0 ± 4393.20	20181.9 ± 7660.2	37303.2 ± 13970.9d
T 1/2z	7.0 ± 1.9	5.4 ± 2.1	6.4 ± 0.8	8.1 ± 5.4
T max	1.6 ± 1.6	1.4 ± 0.7	3 ± 1.2	0.7 ± 0.3c
C max	3797.0 ± 727.7	2203.9 ± 1156.1	2398.4 ± 708.9	8349.5 ± 3969.4dc
Rhein
AUC 0-t	31571.7 ± 8961.8	13308.8 ± 8524.4a	61966.5 ± 18335.3b	38030.3 ± 22227.7dc
T 1/2z	4.1 ± 1.5	6.5 ± 2.3	4.8 ± 0.8	20.8 ± 10.0dc
T max	0.8 ± 0.2	6.5 ± 2.3a	1.7 ± 0.7b	1.6 ± 1.6d
C max	11807.5 ± 2771.1	2204.3 ± 353.4a	9245.7 ± 1284.0b	11113.2 ± 5342.5d
Chrysophanol
AUC 0-t	29977.3 ± 7570.0	15682.3 ± 5428.0a	28337.7 ± 8880.5b	26358.6 ± 4312.1d
T 1/2z	5.7 ± 1.5	4.8 ± 1.0a	4.7 ± 10.0	6.9 ± 4.5
T max	1.4 ± 0.7	3.3 ± 3.2a	1.5 ± 0.6b	1.8 ± 1.7
C max	7766.1 ± 2796.1	2018.9 ± 1227.5a	4153.7 ± 2621.4	4318.1 ± 2458.9
Rheochrysidin
AUC 0-t	867.505 ± 867.5	3665.5 ± 1693.3a	3032.0 ± 1011.6	1510.264 ± 727.3d
T 1/2z	6.1 ± 1.0	4.3 ± 1.2	4.3 ± 1.6	9.4 ± 1.5dc
T max	1 ± 0.7	1.9 ± 1.5	2.2 ± 1.4	1.5 ± 0.6
C max	197.5 ± 64.0	728.8 ± 387.4a	489.8 ± 176.2	172.9 ± 110.7d
Naringin
AUC 0-t	353.0 ± 261.0	6296.0 ± 1271.2a	5449.1 ± 2183.0	2188.5 ± 752.5dc
T 1/2z	6.4 ± 0.8	4.1 ± 0.7a	5.4 ± 1.3	5.8 ± 5.8
T max	0.8 ± 0.2	1.8 ± 1.7	2.3 ± 1.3	2.5 ± 1
C max	125.6 ± 121.0	806.2 ± 730.9a	682.4 ± 380.4	257.6 ± 26.4d
Naringenin
AUC 0-t	3064.2 ± 829.9	6344.8 ± 1199.2a	3051.6 ± 932.6b	3926.0 ± 1014.2d
T 1/2z	7.368 ± 1.5	4.5 ± 1.7	10.9 ± 3.4b	7.8 ± 1.4
T max	0.9 ± 0.7	2.3 ± 1.0	1.6 ± 1.6	0.6 ± 0.1d
C max	634.2 ± 228.6	805.3 ± 113.8	488.9 ± 316.7	581.1 ± 161.7
Magnolol
AUC 0-t	143.8 ± 35.0	34.8 ± 8.0a	69.4 ± 14.8	64.2 ± 10.8
T 1/2z	9.0 ± 6.2	10.6 ± 1.4	13.9 ± 2.5	24.5 ± 9.1d
T max	0.7 ± 0.3	0.8 ± 0.8	3.5 ± 1b	0.5 ± 0.3c
C max	30.6 ± 19.8	5.3 ± 1.7a	5.5 ± 0.9	12.8 ± 3.1

Rats were randomly divided into SOG₁ and three model groups (MG_1, MG_2, and MG₃), and orally dosed with Dachengqi decoction (DCQD) (10 g/kg). Blood samples were collected via the tail vein at 10 min, 20 min, 40 min, 1 h, 2 h, 4 h, 8 h, 12 h, and 24 h after a single dose of DCQD to detect its main components. The pharmacokinetic parameters were calculated with pharmacokinetic statistic software DAS2.0.1. SOG₁: Sham-operated group with the dosing time at 4 h after operation; MG₁, MG₂, and MG₃: Rats were dosed orally with DCQD at 4 h, 12 h, and 24 h after acute pancreatitis induction, respectively. Data are presented as the mean ± SD (n = 6). MG₁ vs SOG₁.

^aP < 0.05; MG₂ vs MG₁.

^bP < 0.05; MG₃ vs MG₂.

^cP < 0.05; MG₃ vs MG₁.

^dP < 0.05. DCQD: Dachengqi decoction.