Calpain-2 activity promotes aberrant endoplasmic reticulum stress-related apoptosis in hepatocytes

Figure 5 Dithiothreitol promotes the accumulation of calpain-2 in the endoplasmic reticulum of BRL-3A cells. After treatment with 2.0 mmol/L dithiothreitol for varying time periods, the cells were labeled with endoplasmic reticulum-tracker red dye, fixed, permeabilized, followed by staining with fluorescent-anti-calpain-2 (green). The cells were examined by fluorescent microscopy, scale bars, 25 μm. Data are representative images (magnification 200 ×) of each group of cells from three separate experiments.