Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice

doi:10.3748/wjg.v25.i5.584

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Feb 7, 2019 (publication date) through May 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Feb 7, 2019; 25(5): 584-599
Published online Feb 7, 2019. doi: 10.3748/wjg.v25.i5.584

Table 2 PCR primers for gene knockout mouse genotyping and corresponding explanations

Primer sequence (5’-3’)	Results	Description
Cldn7-FRTtF2: CCTGGGATCTGATCTGGGTG	wt = 600 bp, fln = none; fl = 756 bp, Null = none	Check if flper is reorganized
Cldn7-FRTtR2: GGCAGGTAGCCTTAGGATGG	wt = 600 bp, fln = none; fl = 756 bp, Null = none	Check if flper is reorganized
ZMK2F4: GCATCGCATTGTCTGAGTAGGTG	wt = none, fln = 515 bp; fl = none, Null = none	Check if Neo is deleted completely
Cldn7-FRTtF2: CCTGGGATCTGATCTGGGTG	wt = none, fln = 515 bp; fl = none, Null = none	Check if Neo is deleted completely
Cldn7-loxPtF2: CTTGGGAGACATCAGGTCCG	wt = 512 bp, fln = 630 bp; fl = 630 bp, Null = none	Homozygous identification
Cldn7-loxPtR2: GAGGCAATAGGCCCAAGGAG	wt = 512 bp, fln = 630 bp; fl = 630 bp, Null = none	Homozygous identification
Cldn7-FRTtF2: CCTGGGATCTGATCTGGGTG	wt = none, fln = none; fl = none, Null = 640 bp	Check if Cre mediated recombination occurs
Cldn7-loxPtR2: GAGGCAATAGGCCCAAGGAG	wt = none, fln = none; fl = none, Null = 640 bp	Check if Cre mediated recombination occurs
Cre-up: GCCTGCATTACCGGTCGATGC	T: 481 bp; W: none	Detect Cre
Cre-low: CAGGGTGTTATAAGCAATCCC	T: 481 bp; W: none	Detect Cre

Citation: Xu C, Wang K, Ding YH, Li WJ, Ding L. Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice. World J Gastroenterol 2019; 25(5): 584-599
URL: https://www.wjgnet.com/1007-9327/full/v25/i5/584.htm
DOI: https://dx.doi.org/10.3748/wjg.v25.i5.584