Reduced microRNA 375 in colorectal cancer upregulates metadherin-mediated signaling

Figure 2 MicroRNA 375 regulates metadherin-mediated BRAF-MAPK signaling in colorectal cancer cell lines. A: Western blot analysis of KRAS, BRAF, mitogen-activated protein kinase 3/1 (MAPK3/1), pMAPK3/1 and β catenin (CTNNB1) expression levels in colorectal cancer (CRC) cells. Except for KRAS; BRAF, MAPK3/1, pMAPK3/1 and CTNNB1 expression levels were downregulated on microRNA 375 (MIR375) mimic and siMTDH transfection in HCT116 cells; B: Western blot analysis of KRAS, BRAF, MAPK3/1, pMAPK3/1 and CTNNB1 expression levels in CRC cells. Except for KRAS; BRAF, MAPK3/1, pMAPK3/1 and CTNNB1 expression levels were downregulated on MIR375 mimic and siMTDH transfection in Caco2 cells. Five independent experiments were performed with duplicates; C: Effects of MIR375 and siMTDH on cell viability in HCT116, Caco2, and SW480 cells. Cell viability was determined by MTT assay. Three independent experiments were performed with duplicates and P values were calculated using Student’s t-test (^aP < 0.05, ^bP < 0.01, ^cP < 0.001). ns: Not significant; MTDH: Metadherin; CTNNB1: β catenin; MAPK3/1: Mitogen-activated protein kinase 3/1; MIR375: microRNA 375; CRC: Colorectal cancer.