Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Nov 14, 2019; 25(42): 6311-6321
Published online Nov 14, 2019. doi: 10.3748/wjg.v25.i42.6311
Figure 5
Figure 5 Overexpression of twinfilin 1 abolishes the effects of loss of miR-30c. A and B: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to determine the expression levels of twinfilin 1 (TWF1). C: Western blot was used to determine the expression levels of TWF1. D and E: CCK-8 assays were used to determine the cell proliferation of different groups. F and G: The apoptosis and cell cycle distribution of pancreatic cancer cells in different groups were investigated by flow cytometry. H: RT-qPCR detection of the expression of TWF1 in tissues collected at The First Affiliated Hospital of Zhengzhou University. I: Negative correlation between the expression levels of miR-30c and TWF1 in pancreatic cancer tissues. The data are presented as the mean ± standard deviation. All experiments were performed at least three times. P < 0.05 was considered statistically significant. eP < 0.001 vs nontumor group; cP < 0.05 vs miR-30c + TWF1 group. TWF1: Twinfilin 1.