MicroRNA-30c inhibits pancreatic cancer cell proliferation by targeting twinfilin 1 and indicates a poor prognosis

Figure 2 MiR-30c inhibits the growth of pancreatic cancer cell lines in vitro. A: Expression levels of miR-30c in five human pancreatic cancer cell lines and human pancreatic ductal epithelial cell line (HPDE). B: Expression levels of miR-30c in cells transfected with control, miR-30c mimics, or miR-30c inhibitors. C: CCK-8 assays showed distinct differences in proliferation after manipulation of miR-30c in Mia PaCa-2 cells. D: CCK-8 assays showed distinct differences in proliferation after manipulation of miR-30c in BxPC-3 cells. E: Flow cytometry assays were performed to detect the apoptotic rates of cells after transfection. F: Flow cytometric analysis was performed to detect the cell cycle distribution of cells after transfection. All experiments were performed at least three times. The data are presented as the mean ± standard deviation. P < 0.05 was considered statistically significant. ^aP < 0.05 vs control group; ^bP < 0.01 vs control group; ^eP < 0.001 vs control group; ^cP < 0.05 vs HPDE; ^dP < 0.01 vs HPDE. HPDE: Human pancreatic ductal epithelial cell line.