Circular RNA PIP5K1A promotes colon cancer development through inhibiting miR-1273a

Figure 4 Effect of miR-1273a overexpression on circPIP5K1A upregulation. A: Binding site of miR-1273a and circPIP5K1A; B: Expression of AP-1, IRF-4, CDX-2, and Zic-1 in COLO320DM cells transfected with circPIP5K1A vector, miR-1273a vector, or co-transfection of circPIP5K1A vector and overexpressing miR-1273a vector, respectively, by western blotting. ^aP < 0.05, ^bP < 0.01 vs control group; ^cP < 0.05, ^dP < 0.01 vs circPIP5K1A group. Values are mean ± SD. circPIP5K1A: Circular RNA PIP5K1A; AP-1: Activator protein 1; IRF-4: Interferon regulating factor 4; CDX-2: Caudal type homeobox 2; Zic-1: Zinc finger of the cerebellum 1.