Ex vivo effect of vascular wall stromal cells secretome on enteric ganglia

Figure 4 Effect of porcine vascular wall mesenchymal stromal cells supernatants on the number of neurons (left white bars) and glial cells (gray right bars) exposed to lipopolysaccharide 1 µg/mL. A: The number of neurons in guinea pig enteric ganglia did not change significantly upon any of the treatment tested, whereas co-treatment with conditioned medium (CM) and lipopolysaccharide 1 µg/mL (LPS1) (CM+LPS1) increased the number of glial cells compared to control (CTRL) and LPS1-treated ganglia (+13.9%, ^bP < 0.001; +16.5%, ^dP < 0.01, respectively); B: The number of neurons in pig enteric ganglia did not change as a result of any of the treatment tested. Conversely glial cell number was higher in the CM group compared to control (+13.6%, ^aP < 0.05) and LPS1 (+20.2%, ^cP < 0.05). CM+LPS1 co-treatment increased the number of glial cells compared to LPS1-treated ganglia (+14.2, ^eP < 0.05). LPS1: Lipopolysaccharide 1 µg/mL; CM: Conditioned medium. GFAP: Glial fibrillary acidic protein.