Tenofovir is a more suitable treatment than entecavir for chronic hepatitis B patients carrying naturally occurring rtM204I mutations

Table 2 Measurement of melting temperatures of hepatitis B virus wild type (YMDD) and rtM204I variants (YIDD) by LNA real-time PCR

Sample (copies, number of samples n)	Measured Tm (°C) at channel
	FAM			HEX
	Min	Max	Mean ± SD (number of positives)	Min	Max	Mean ± SD (number of positives)
YIDD positive control DNA (24-2400000, n = 48)	51.3	52.2	51.7 ± 0.2 (48, 100%)	(-)	(-)	(-)
WT positive control DNA (24-2400000, n = 48)	(-)	(-)	(-)	61.9	63.3	62.6 ± 0.4 (46, 95.8%)
YIDD and WT standard mixtures (24-2400000, n = 280)	50.1	52.6	51.5 ± 0.3 (280, 100%)	61.9	64.0	63.0 ± 0.4 (280, 100%)
YIDD and WT standard mixtures (24, n = 56)	49.9	51.9	51.2 ± 0.5 (45, 80.3%)	60.9	63.8	63.0 ± 0.5 (44, 78.6%)
YIDD of clinical samples in duplicate (82-180000, n = 17)	49.6	51.9	51.3 ± 0.5	(-)	(-)	(-)
YMDD of clinical samples in duplicate (65-13000000, n = 397)	(-)	(-)	(-)	61.1	64.0	62.8 ± 0.3

¹The variants were identified by direct sequencing of PCR products. (-) indicates no significant melting temperature; SD: standard deviation; T_m: Melting temperature.