Towards a standard diet-induced and biopsy-confirmed mouse model of non-alcoholic steatohepatitis: Impact of dietary fat source

Table 1 Plasma and liver biomarkers in ob/ob mice fed amylin liver non-alcoholic steatohepatitis (AMLN) or Gubra amylin non-alcoholic steatohepatitis (GAN) diet for 8-16 wk

Group	Weeks on diet	n	ALT (U/L)	AST (U/L)	Plasma TG (mmol/L)	Plasma TC(mmol/L)	Liver lipid mass (% of liver weight)
Chow C57	8	6	115 ± 60	192 ± 77	1.7 ± 0.2	3.8 ± 0.2	3.5 ± 0.4
	12	6	67 ± 10	93 ± 16	1.7 ± 0.1	3.5 ± 0.1	4.9 ± 0.7
	16	6	61 ± 18	82 ± 18	2.2 ± 0.2	3.8 ± 0.3	3.6 ± 0.4
GAN ob/ob	8	4	913 ± 113a	663 ± 37a	1.2 ± 0.2	11.8 ± 0.9a	31.6 ± 1.3a
	12	5	959 ± 93a	660 ± 52a	1.4 ± 0.1	12.4 ± 1.3a	33.3 ± 0.7a
	16	5	868 ± 102a	674 ± 25ad	1.5 ± 0.2a	14.3 ± 0.8ad	28.4 ± 1.4ad
AMLN ob/ob	16	6	654 ± 39a	399 ± 23a	1.0 ± 0.1a	11.0 ± 0.4a	35.4 ± 0.8ad

^aP < 0.05 vs corresponding feeding period in chow-fed C57BL/6J (Chow C57) mice,

^dP < 0.05 vs corresponding feeding period in amylin liver non-alcoholic steatohepatitis (AMLN) ob/ob mice. TC: Total cholesterol; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; TG: Total triglycerides; AMLN: Amylin liver non-alcoholic steatohepatitis diet; GAN: Gubra amylin non-alcoholic steatohepatitis diet; NASH: Non-alcoholic steatohepatitis.