Fate plasticity in the intestine: The devil is in the detail

Table 2 Concepts leading to difficulties in ascribing behaviour to cell types in the intestine

CreER and Tamoxifen	Toxicity
	Off-target effects
Incongruity between reporter expression and protein expression	Regional differences
	Chronicity of reporter stability
	Reporter and mRNA expression differences
Inconsistent injury models	Intestinal specific effects including incomplete cell type eradication e.g., diphtheria toxin mediated cell death
	Off-target whole body effects e.g., irradiation
	Representative of “homeostatic regeneration”
	Different cell-type responses to different injuries
Laboratory differences	Microbiota
	Diet
	Area of intestine examined
	Strain differences between laboratories due to inbreeding