Sp1 contributes to overexpression of stanniocalcin 2 through regulation of promoter activity in colon adenocarcinoma

Figure 6 The transcription factor Sp1 contributed to the overexpression of STC2 in colon cancer cells. A: Overexpression of Sp1 in HEK293T and NCM460 cells. Western blotting was used to evaluate the protein expression of STC2; B: The quantity of panel A protein density with Quantity One software, ^cP < 0.001 vs Myc-Vector of individual cell group; C: Knocking down the expression of Sp1 with pSuper knockdown system in HT29 and SW480 cell lines. The protein levels of STC2 were evaluated with western blotting assay; D: The quantity of panel C protein density with Quantity One software. ^cP < 0.001 vs sh-Vector of individual cell group. STC2: Stanniocalcin 2.