Functional role of long non-coding RNA CASC19/miR-140-5p/CEMIP axis in colorectal cancer progression in vitro

Figure 2 Role of cancer susceptibility 19 in the proliferation and migration of colorectal cancer cells. A: Expression of cancer susceptibility 19 (CASC19) in colorectal cancer (CRC) cells transfected with siCASC19 or CASC19-p and their NCs was quantified by quantitative real-time PCR. B: Growth curves of HCT-116 and SW480 cell lines that were transfected with CASC19-p or siCASC19 were analyzed using the Cell Counting Kit-8 assay. C: Western blot was used to determine the expression of caspase 3, caspase 7, and PARP in HCT-116 and SW480 cells that were transfected with either CASC19-p or siCASC19. The grayscale value of proteins was measured with Image J. D: Cell apoptosis rate was detected by flow cytometry after transfection with CASC19-p or siCASC19. E: The proportion of cells in each cell cycle phase was detected using flow cytometry after cells were transfected with CASC19-p or siCASC19. Data are reported as the mean ± SD. ^aP < 0.05. CASC19: Cancer susceptibility 19; CRC: Colorectal cancer.