Relaxin influences ileal muscular activity through a dual signaling pathway in mice

Figure 3 Effects of relaxin, in the absence or in the presence of oxadiazolo[4,3-a]-quinoxalin-1-one and 9-cyclopentyladenine, on membrane potential recorded from smooth muscle cell of the mouse ileum. Typical tracings of membrane potential from four different single ileal SMCs. A-D: Irregular waves of MP recorded in control condition. RLX addition (arrow) induced a persistent hyperpolarization occurring right after about 2 min from its application (A); The hyperpolarization caused by RLX was reduced in the presence of ODQ (B), 9CPA (C) and was abolished when both blockers were concomitantly present in the bath solution (D). Breaks in X-axis (and consequently in the MP traces) indicate the end of each episode, RLX: Relaxin; ODQ: Oxadiazolo[4,3-a]-quinoxalin-1-one; 9CPA: 9-cyclopentyladenine.