Near-infrared photoimmunotherapy of pancreatic cancer using an indocyanine green-labeled anti-tissue factor antibody

Figure 5 Photoimmunotherapeutic effects on BxPC-3 xenografts and body weight change in mice. A: Tumor volume change is expressed as the ratio of the volume on the indicated day and volume at 1 d before the start of near-infrared photoimmunotherapy (NIR-PIT). Tumors treated by NIR-PIT (red) showed significantly reduced growth rates compared to those, that received no treatment (light blue), NIR light exposure alone (pink), indocyanine green-labeled anti-tissue factor (TF) antibody 1849 (1849-ICG) alone (green) and anti-TF antibody 1849 (anti-TF 1849) alone (blue), for 27 d after the treatment start date. Values shown represent mean ± SD, ^aP < 0.05, NIR-PIT vs other groups (n = 12 for each group except n = 11 in anti-TF 1849 group); B: Significantly prolonged survival was observed in NIT-PIT group vs other groups, ^bP < 0.05, by Gehan-Breslow-Wilcoxon test (n = 12 for each group except n = 11 in anti-TF 1849 group); C: The average body weight did not differ significantly among all 5 groups of mice. Red arrowhead indicates the day of administration of 1849-ICG or Anti-TF 1849 and purple arrowheads indicate the day of NIR exposure. Anti-TF 1849: Anti-tissue factor antibody 1849; 1849-ICG: Indocyanine green-labeled anti-tissue factor antibody 1849; NIR: Near-infrared; PIT: Photoimmunotherapy.