Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer

Figure 4 Sirtuin 3 is a transcriptional target of zinc finger E-box binding homeobox-1. A: There is a specific zinc finger E-box binding homeobox-1 (ZEB1) binding element (Z-box: CAGGTA) in the promoter region of sirtuin 3 (SIRT3); B: ZEB1 suppressed SIRT3 promoter luciferase activity in a dose-dependent manner; C: The Z-box in the SIRT3 promoter was mutated from CAGGTA to GTGGTA to confirm that ZEB1 specifically regulated SIRT3 promoter activity; D: ZEB1 had a slight impact on mutated SIRT3 promoter luciferase activity, suggesting that ZEB1 regulated SIRT3 expression by Z-box specific binding. ^aP < 0.05, and ^bP < 0.01 vs NC or mock group. ZEB1: Zinc finger E-box binding homeobox-1; SIRT3: Sirtuin 3.