Zinc finger E-box-binding homeobox 1 mediates aerobic glycolysis via suppression of sirtuin 3 in pancreatic cancer

Figure 1 Zinc finger E-box binding homeobox-1 mediates aerobic glycolysis in pancreatic cancer. A: Zinc finger E-box binding homeobox-1 (ZEB1) knockdown efficiency was assessed by quantitative real-time polymerase chain reaction in PANC-1 and MIA PaCa-2 cells; B: Immunoblot with ZEB1 antibody confirmed that ZEB1 was effectively downregulated in PANC-1 and MIA PaCa-2 cells; C: ZEB1 knockdown decreased glycolysis, as reflected by extracellular acidification rate measurements; D: ZEB1 knockdown increased mitochondrial respiration, measured by oxygen consumption rate; E and F: Decreased ZEB1 expression in PANC-1 and MIA PaCa-2 cells decreased reactive oxygen species generation; G and H: Downregulation of ZEB1 increased mitochondrial membrane potential in PANC-1 and MIA PaCa-2 cells. ^aP < 0.05, and ^bP < 0.01 vs NC or mock group. Error bars indicate mean ± SD. ZEB1: Zinc finger E-box binding homeobox-1; ECAR: Extracellular acidification rate; OCR: Oxygen consumption rate.