Mucosal adhesion and anti-inflammatory effects of Lactobacillus rhamnosus GG in the human colonic mucosa: A proof-of-concept study

Figure 4 Lactobacillus rhamnosus GG dose-finding study in the ex vivo organ culture experimental model. A: Mucosal Lactobacillus rhamnosus GG (LGG) quantification in distal colon bioptic samples after incubation with a regular (6 × 10⁶ CFU), double or 5-times dose of LGG. ^aP < 0.05 vs regular dose (n = 8 per group); B: TNFα mRNA quantification in distal colon bioptic samples incubated with a regular (1:10), double (2:10) or 5-times (5:10) dose of LGG conditioned media (CM) (n = 10 per group); C: IL-17 mRNA quantification in distal colon bioptic samples incubated with a regular (1:10), double (2:10) or 5-times (5:10) dose of LGG CM. Mean ± standard error is represented. ^aP < 0.05 vs regular dose. TNFα: Tumor necrosis factor alpha; IL: Interleukin; LGG: Lactobacillus rhamnosus GG; CM: Conditioned media.