Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension

doi:10.3748/wjg.v24.i38.4356

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Oct 14, 2018 (publication date) through Apr 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 14, 2018; 24(38): 4356-4368
Published online Oct 14, 2018. doi: 10.3748/wjg.v24.i38.4356

Table 1 Nucleotide sequences of forward and reverse primers

Gene	Forward primer (5´-3´)	Reverse primer (5´-3´)	Product length (bp)
eNOS	5´-AAGTGGGCAGCATCACCTAC-3´	5´-GCCTGGGAACCACTCCTTTT-3´	211
iNOS	5´-CTCACTGGGACTGCACAGAA-3´	5´-TGTTGAAGGGTGTCGTGAAA-3´	128
PDE-5	5´-GCGGAGGAAGAAACAAGGGA-3´	5´-ATCGGCAAAGAACCTCGTGT-3´	196
sGCa1	5´-GCCCCACGACATACAGGTTA-3´	5´-GCGGCTCACTAATCTACCCC-3´	229
sGCb1	5´-AATTACGGTCCCGAGGTGTG-3´	5´-ACCAGCATTGAGGTTGAGGAC-3´	147
18sRNA (reference)	5´-GTAACCCGTTGAACCCCATT-3´	5´-CCATCCAATCGGTAGTAGCG-3´	151
srsf4 (reference)	5´-GGTTCTGGACGCAGTGGATA-3´	5´-CTCCTTCGTTTTTGCGTCCC-3´	193

Citation: Schaffner D, Lazaro A, Deibert P, Hasselblatt P, Stoll P, Fauth L, Baumstark MW, Merfort I, Schmitt-Graeff A, Kreisel W. Analysis of the nitric oxide-cyclic guanosine monophosphate pathway in experimental liver cirrhosis suggests phosphodiesterase-5 as potential target to treat portal hypertension. World J Gastroenterol 2018; 24(38): 4356-4368
URL: https://www.wjgnet.com/1007-9327/full/v24/i38/4356.htm
DOI: https://dx.doi.org/10.3748/wjg.v24.i38.4356