Daikenchuto (Da-Jian-Zhong-Tang) ameliorates intestinal fibrosis by activating myofibroblast transient receptor potential ankyrin 1 channel

Figure 1 Chronic 2, 4, 6-trinitrobenzenesulfonic acid-induced colitis models of wild-type and transient receptor potential ankyrin 1-knockout mice. Chronic colitis was induced by weekly intrarectal injection of TNBS for six weeks. Immediately after the final TNBS treatment, a DKT enema was administered daily for one week. A: schematic presentation of TNBS injection and DKT enema; B: Hematoxylin–eosin (HE)- and Masson's trichrome (MT)-stained tissues from the vehicle control, TNBS and TNBS + DKT groups of WT and TRPA1-/- mice. TNBS: 2, 4, 6-trinitrobenzenesulfonic acid; WT: Wild-type; TRPA1: Transient receptor potential ankyrin 1; TRPA1-/-: Transient receptor potential ankyrin 1-knockout; DKT: Daikenchuto; HE: Hematoxylin-eosin; MT: Masson's trichrome.