Copyright
©The Author(s) 2018.
World J Gastroenterol. Sep 21, 2018; 24(35): 4028-4035
Published online Sep 21, 2018. doi: 10.3748/wjg.v24.i35.4028
Published online Sep 21, 2018. doi: 10.3748/wjg.v24.i35.4028
Table 2 Touchdown-PCR setup
| Step | Temperature (°C) | Time | Note |
| 1 | 94 | 3 min | - |
| 2 | 94 | 20 s | - |
| 3 | 64 | 30 s | -0.5 °C per cycle |
| 4 | 72 | 35 s | Repeat steps 2-4 for 12 cycles |
| 5 | 94 | 20 s | - |
| 6 | 58 | 30 s | - |
| 7 | 72 | 35 s | Repeat steps 5-7 for 25 cycles |
| 8 | 72 | 2 min | - |
| 9 | 4 | Hold |
- Citation: Idrizaj E, Garella R, Castellini G, Mohr H, Pellegata NS, Francini F, Ricca V, Squecco R, Baccari MC. Adiponectin affects the mechanical responses in strips from the mouse gastric fundus. World J Gastroenterol 2018; 24(35): 4028-4035
- URL: https://www.wjgnet.com/1007-9327/full/v24/i35/4028.htm
- DOI: https://dx.doi.org/10.3748/wjg.v24.i35.4028