Total flavone of Abelmoschus manihot suppresses epithelial-mesenchymal transition via interfering transforming growth factor-β1 signaling in Crohn’s disease intestinal fibrosis

Figure 6 Total flavone of Abelmoschus manihot inhibited transforming growth factor-β1-induced activation of MAPK signaling pathway. A: The effect of TFA on TGF-β1-induced activation of MAPK signaling was evaluated by western blotting; B: The expression of MAPK signaling was examined by western blotting after transfection with MAPK inhibitors; B and C: The effects of MAPK inhibitors or TFA combined with MAPK inhibitors on TGF-β1-induced activation of Smad signaling were evaluated by western blotting (B) and qRT-PCR (C) assays. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 compared with that of non-TFA treated group, ^dP < 0.05, ^eP < 0.01 compared with that of TGF-β1 (10 ng/mL) treated group. TFA: Total flavone of Abelmoschus manihot; TGF: Transforming growth factor.