Downregulation of Hes1 expression in experimental biliary atresia and its effects on bile duct structure

Figure 4 Expression levels of Notch signaling components in the liver of rhesus rotavirus-inoculated mice. The mRNA levels of Notch signaling components, including Jag1, Dll1, Notch1, Notch2 and Hes1, were detected using quantitative real-time PCR with appropriate primers at day 7 and day 14 after RRV inoculation. A: The relative expression levels compared with the normal control are shown for day 7 (^aP < 0.05; ^bP < 0.01; n = 6 in the control group and n = 10 in the RRV group); B: day 14 (^cP < 0.001); C: The levels of the Hes1 protein and the expression of the transcriptional coregulator RBP-Jκ were detected by Western blot (n = 2 or 3 in each group; the proteins were mixed and loaded).