Total polysaccharides of the Sijunzi decoction attenuate tumor necrosis factor-α-induced damage to the barrier function of a Caco-2 cell monolayer via the nuclear factor-κB-myosin light chain kinase-myosin light chain pathway

Figure 8 Effects of total polysaccharides of the Sijunzi decoction on the nuclear factor-κB signaling pathway. A: TPSJ upregulated the activity of the NF-κB transcription factor p65; B: A representative western blot of NF-κB p65 in TNF-α-damaged Caco-2 cells treated with TPSJ; C: Quantification of the amounts of p65 relative to PCNA. Data are shown as the means ± standard errors of at least three independent experiments ^bP < 0.01 vs control Caco-2 cells; ^cP < 0.05, ^dP < 0.01 vs TNF-α-damaged Caco-2 cells). TPSJ: Total polysaccharides of the Sijunzi decoction; NF: Nuclear factor; TNF: Tumor necrosis factor.